tobacco hornworm host plants horrors; squeamish gardeners need not apply Musings on the Garden: Tomato

Long-term use of heroin can cause physical, psychological and behavioral changes in the addict

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Retrieved 7 March 2024

Case Studies Case 1: TEV Won't Cut Protein: 45 kDa enzyme Construct: His6-TEV site-Target Problem: No cleavage after 24 hours with excess TEV Diagnosis: Crystal structure of homolog showed N-terminus buried in a groove TEV site was inaccessible to protease Solution: Redesigned: His6-(GGGGS)-TEV site-(GGGGS)-Target Cleavage now complete in 4 hours Case 2: Thrombin Destroys the Protein Protein: 30 kDa signaling domain Construct: His6-Thrombin site-Target Problem: Multiple bands after cleavage

Elsewhere from 1982 to 1988

I give this dupe a 8.75/10 (and i gave RT a 7.5/10 just because its so harsh and difficult to wear)