Extraction Procedure, Method 1 The internal standards, in 0.01 N aqueous HCl, 100 L of a mixture of anabasine-d 4 , (100 ng/ml) anatabine-d 4 (100 ng/ml), nicotelline-d 8 (10 ng/ml), anatalline-d 4 (4 ng/ml) and anatalline metabolite-d 4 (150 ng/ml), were added to 0.5 ml sample of urine
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