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These primers were designed based on the human mitochondrial sequence procured from the National Centre of Biotechnology Information (NCBI) database ( The oligonucleotide primers were synthesized by Microsynth Seqlab in Germany, as detailed in Table 7

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Unlike cigarettes and alcohol, which dominated past generations of celebrities, cannabis generally has fewer long-term harms when used responsibly and is coming up more often in mainstream celebrity culture

In addition, more than 80% of adolescents said that school education on e-cigarettes was limited and superficial, and the current form of education on e-cigarettes mainly was based on lectures and videos

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