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In this assay, the 30 min incubation duration was chosen to be optimal because of the inherently cytotoxic nature of CuCl 2 and Cu(OAc) 2 towards HaCaT keratinocytes, as they were observed to cause substantial cell death at a duration longer than 30 mins

This is especially valuable in a category like GHK-Cu peptide serum, where the product can be positioned as premium and science-led, but the brand still needs to validate whether its audience responds to the concept, price point, and packaging presentation

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While the science in animals is intriguing, the lack of controlled human trials especially in women makes their use highly uncertain and potentially risky

The advantage is that measurement precision is very forgiving at this concentration