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For phospho-protein staining, cells were treated with the indicated smoking-related stimuli for an appropriate amount of time, after which they were resuspended in 100 L of culture medium and fixed with an equal volume of BD Cytofix fixation buffer for 15 min at 37 C, resuspended in 150 L of BD Cytoperm Solution III for 30 min on ice, and resuspended in PBS containing 2% FBS and PE Mouse Anti-H2AX (pS139) (1:50) or PE Mouse anti-NF-B p65 (pS529) (1:50) for 20 min at room temperature

190w Share Info Candlelit Connection Under Starry Skies Model: DaVinci2 Size: 1152 X 864 (1.00 MP) Used settings: Prompt : *"Realistic illustration of two intertwined souls forming in the shape of smoke, gently rising from a candle

10.1038/sj.npp.1300450 24

However, other animal studies did not observe this type of age-dependent differences

Tobacco Control, 23 : ii36-ii40