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Antioxidants, such as L-ascorbic acid, L-histidine, -tocopherol, -carotene and astaxanthin may rescue the motor neurons injured by SOD1 inhibition

Extracts were cleared by centrifugation at 15,000 g and 4 C for 15 min, boiled at 95 C for 5 min and finally analyzed by SDS-PAGE and Western blots using antibodies against GFP, -tubulin as loading control, and HA-tag when required

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