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Factors such as rising temperatures, irregular rainfall, droughts, and floods in major tobacco-growing regions are increasingly impacting crop yields, leaf quality, and curing processes, resulting in an inconsistent supply of raw materials

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100 L of native TMV and mutant TMVconcentrations determined by Lowry assay (0.50.015 g/ml)were serially diluted with sample extraction buffer (0.009 M Na 2 SO 4 , 2% (v/v) Polyvinylpyrrolidone (PVP) 40 k, 0.2% (w/v) Powdered egg (chicken) albumin, 0.003 M NaN 3 , 0.2% (v/v) Tween-20 in washing buffer) was then added to each well and incubated at RT for 2 h

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doi: 10.2105/ajph.89.12.1837

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