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Nicotine is named after 16thcentury French scholar Jean Nicot, who promoted its medicinal qualities, and in the 1570s Spanish doctor Nicolas Monardes recommended tobacco as a cure for 36 ailments including toothache, worms, halitosis and cancer
Cells were fixed in 2.5% glutaraldehyde in 200 mM sodium phosphate buffer (pH 7.4), and then pre-equilibrated three times for 3 min with 4 mM K 2 HPO 4 (pH 9), prior to staining for 15 min with 0.05% (w/v) Aniline Blue (Water Blue
I think we all took that artistic approach to tin packaging for granted, assuming it would never go away
When dry, the leaves were crushed
By expressing a GFP-fusion in protoplasts, and by fractionation of vesicles, these authors tried to corrobate a vacuolar localisation
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