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In general, higher air pollution exposure was associated with lower methylation of specific CpG sites in GC ( 6 and VB 12 ) and iron, and several prior studies ( 12 , causing the inactivation of these micronutrients ( 12 deficiencies also reduce DNA methylation via homocysteine increase, ultimately aggravating the effect of air pollution on DNA methylation ( 6 and VB 12 levels, and increased homocysteine levels ( 4.4 Limitation The findings of this study need to be interpreted in consideration of the following limitations