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4,539,709 reads with an average length of 100nt aligned to the mitogenome and the resulting depth of coverage (DOC) chart revealed discrete regions with moderate to high DOC separated by spans of very low to non-existent coverage (Figures 1A & B, Additional file1: Figure S1)

Depth: Approx

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Even today, many high-profile sources list the toxic dose of nicotine (the LD50 or the dose that will kill about half of people exposed) as between 30 and 60 mg

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