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After incubation overnight, the primary antibody was removed, the membrane then was washed five time for 5 min each time in TBST, followed by addition of the secondary antibody (Goat Anti-Rabbit IgG H&L (HRP) for 120 min

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8 The rapid intensification of cases over a four-month period led the Centers for Disease Control and Prevention (CDC) and the U.S

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