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The symptom expression was recorded and the association of the virus was confirmed by direct antigen coated enzyme-linked immunosorbent assay (DAC-ELISA) using antiserum developed from purified virus preparations of FrMV, and RT-PCR using CP-gene specific primers to FrMV, developed in our previous study (Kumar et al., 2015)

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However, the micronucleus assay was positive for both iSENZIA aerosols and cigarette smoke, though iSENZIA aerosols showed much lower genotoxic potency