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Membranes were probed with antibodies for AIF (5318, Cell Signaling, Danvers, MA), EndoG (ab9647, Abcam, Cambridge, MA), NUP98 (2598, Cell Signaling) and -actin (A2228, Sigma-Aldrich), followed by HRP-anti mouse IgG (4741806, KPL Inc.) or HRP-anti-rabbit IgG (4741506, KPL, Gaithersburg, MD) as indicated

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For disposable respirators, the subject cups both hands over the front of the respirator and then inhales sharply