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To isolate LPMCs, the tissues were digested in RPMI (Gibco) supplemented with 20% fetal bovine serum (FBS), 1 mg/mL collagenase IV, and 0.05 mg/mL DNase I at 37 C for 30 min
There were also no data indicating whether or not the respondents were taking any medications associated with ED such as antidepressants or beta blockers
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Furthermore, deaths were divided into subgroups according to the cause
They determined: Smoking lowers the pitch of the voice (fundamental frequency Smoking lowers the maximum phonation time (the length of time you can make sound before needing to breathe again) Smoking may cause voice disorders, such as cancer, although functional disorders (i.e., injury) were not clearly proven Smoking can dry the vocal cord mucosa and irritate the vocal cords Smoking causes vocal cord inflammation Pulmonary function is weakened (breath supply and vocal power) Of note, it is not the tobacco that is the culprit
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