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Physical activity assessment integrated subjective and objective approaches, namely the International Physical Activity Questionnaire-Short Form (IPAQ-SF) to measure seven-day physical activity patterns and mobile phone-based pedometer measures of average daily steps during the study period

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Finally, proteins were detected by ECL reagents (BIO-RAD, USA), and densities of bands were quantified by Image J software

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Collected butts are sent to TerraCycle Canada, where they are recycled into new products