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Its the third leading cause of death worldwide, with smoking being the major risk factor

However, plants may alter metabolic pathways to enhance osmoregulation and improve stress resilience ( In response to heat stress, tobacco plants activate multiple heat stress-responsive genes, typically regulated by heat shock transcription factors (HSFs) ( HSP70 and HSP90 have been extensively studied, and their upregulated expression under heat stress plays a significant role in maintaining cellular homeostasis and enhancing heat tolerance efficiency ( However, genes associated with antioxidative responses, such as SOD and CAT, are also induced under heat-stress conditions

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