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The confirmed year ranges span 20062011 for the S80 II, 20072015 for the V70 III, 20082017 for the XC60 I, and 20092010 for the XC70 II

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The resin was then mixed with target proteins (~ 1.6 mg of ACP-TEV or TEV) contained in coupling buffer (1 mM EDTA, 50 mM TRISHCl, pH 8.8) and gently rocked at 37 C for 2 h