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Analysis of sequencing information Processing of sequencing data According to Barcode sequences and primer sequences for PCR amplification, the data on various samples were separated from the offline data

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2004 Annual Report" (PDF)

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Cleaved products were dA-tailed, ligated with a hairpin adaptor (NEB), treated with USER enzyme (NEB) and amplified by PCR with barcoded universal primers NEBNext Multiplex Oligos for Illumina (NEB), using Kapa HiFi Polymerase (KAPA Biosystems)