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After homogenization, the samples were placed inside the refrigerator for 15 min to aid the precipitation and then centrifuged at 10,000 rpm for 15 min at 4 C to precipitate out the protein The supernatant was used to determine 5-HT and 5-HIAA levels in the cerebral cortex and midbrain samples

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These findings indicate that mitochondrial heterogeneity affects the physiology and metabolism of the body and cognitive functions related to the nervous system, highlighting the importance and urgency of exploring and solving the problem of mitochondrial heterogeneity in ART [46, 128]