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These genes protect cells from oxidative damage by scavenging ROS, enhancing tobaccos stress tolerance ( ZmHSP16.9 , RcHSP17.8 , BcHSP70, and LeHSP21 , can maintain photosynthetic responses by increasing seed germination rates, chlorophyll content, and antioxidant capacity while reducing MDA content and conductivity, thereby significantly enhancing plant heat tolerance apparatus ( CAP2 , seed dormancy delay family gene NtDOG1L-T, and heat shock transcription factor gene BcHsfA1 in tobacco seedlings significantly enhances promoter activity under heat stress conditions, with notable increases in the expression levels of HSPs and heat shock factor genes ( APX2 ) and superoxide dismutase C (SODC), as well as the heat shock network genes