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The isolated oligonucleotides are ligated to adaptors, and after damage-specific immunoprecipitation, the adaptor-ligated oligonucleotides are converted to dsDNA [double-stranded DNA] with an appropriate translesion DNA synthesis (TLS) polymerase, followed by PCR amplification and next-generation sequencing (NGS) to generate genome-wide repair maps
A prohibition would, they say, advance the admirable goal of protecting the health and safety of both players and the fans that emulate themespecially since the 2011 restrictions are no guarantee that tobacco will not end up on television
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