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Additionally, a mutation of S121 3.39 K (superscript indicates residues numbers according to the BallesterosWeinstein scheme 16 ) at the putative allosteric Na + binding site was introduced to improve the homogeneity and thermostability of H 3 R as described in several GPCR structures determination 17,18,19,20,21,22 (Supplementary Fig

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7 Italian anise-flavored liqueur : SAMBUCA Sambuca is an Italian liqueur that is flavored with anise