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The conditions of qPCR were as follows: an initial denaturation at 95 C for 5 min, followed by 40 cycles of 95 C for 30 s, 55 C for 30 s, and 72 C for 30 s, concluding with a final extension at 72 C for 5 min

Time needed: not less than 60-120 minutes

This retrospective cohort study of 430,016 adults recruited from a standard health-screening program since 1994, with 11,031 deaths identified as of 2008

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