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51 As GSH is known to be present in CSF at very low concentrations (~0.2 mol/l in both healthy and PD subjects), 52 relative to brain tissue concentrations (~1 mmol/l), 7 LCModel-calculated Cr concentrations ( C measured ) were corrected ( C corrected ) using the following formula, which assumes negligible contribution of CSF GSH to the total GSH signal: Absolute GSH levels (IU) were calculated by multiplying GSH/Cr ratios by CSF-corrected absolute Cr concentrations (IU)

Cells were trypsinized for 5 min at 37 C, diluted in DMEM, and centrifuged at 400 g for 10 min

maintaining the essential thiol status of proteins and other molecules Stores important amino acids

Antioxidant supplementation during in vitro culture improves mitochondrial function and development of embryos from aged female mice

10.3390/ijms23147977 75 DoctrowS