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and none of the downstream applications remove endotoxin entirely
cloni 10G SUPREME Electrocompetent Cells consistently outperformed “ultra high efficiency” cells from a leading supplier
Rapid extraction of RT-PCR-ready RNA from cultured mammalian cells
mixture of 3 µg of digested bacterial chromosomal DNA and 500 ng of uncut plasmid before Plasmid-Safe DNase treatment
Unit Definition: One Molecular Biology Unit (MBU) of RNase-Free DNase I digests 1 µg of pUC19 DNA to oligodeoxynucleotides in 10 minutes at 37°C under standard assay conditions
E. cloni-10G CLASSIC Electrocompetent Cells, 48 rxns (SixPacks) cell projects and none of the downstreamTable 1. E. cloni Competent Cells for Cloning and Library Construction E. cloni Cell Lines Transformation Efficiency (cfu g pUC DNA) Cloning Methylated DNA BAC, Cosmid Cloning Blue White Screening 10G SUPREME Electrocompetent >4 1010 YES YES YES without IPTG induction 10G ELITE Electrocompetent >2 1010 YES NO YES without IPTG induction 10G CLASSIC Electrocompetent >5 109 YES NO YES without IPTG induction 10GF ELITE Electrocompetent >2 1010 YES NO YES
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