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Choice of efficiency:
ChIP-seq and FFPE and cell free DNA samples
CircLigase ssDNA Ligase ligates ends of ssDNA in the absence of a complementary sequence
The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3)
10G ≥ 1×109 cfu/µg
Exonuclease III, 25,000 U @ 200 U/uL OverExpress C41(DE3) Choice of efficiency:Digest duplex DNA in a 3 to 5 direction from a nick, blunt end, or 3 recessed end, producing stretches of ssDNA. Produce stretches of ssDNA in dsDNA templates containing nicks, blunt ends or recessed 3' ends Protect protruding 3' ends becuase this exonuclease will not start digestion at protruding 3' overhangs Applications Production of intermediates for site directed mutagenesis. 1 3 Production of strand specific radiolabeled probes. 4 Exonuclease
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