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Internal reference standards sulforaphane-d8 (50 M) and sulforaphane-sulforaphane-d8-N-Acetyl-L-cysteine (10 M) were added to each well of the collection plate before eluting the samples with 0.2% AA in methanol

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Low angiotensinogen levels in the brain lead to anxiety-like behaviors and depression-like behaviors, while intracerebroventricular administration of Ang-(17), selective serotonin reuptake inhibitor fluoxetine, enalapril (ACEI) attenuated behavioral changes in transgenic hypertensive rats, as shown by spending a lower percentage of time in the open arms of EPM and decreasing immobility time in FST (25, 38)