RNase I, 1,000 U size:EACH Seven specific deletion mutations (ΔgutQ

Seven specific deletion mutations (ΔgutQ ΔkdsD ΔlpxL ΔlpxMΔpagPΔlpxPΔeptA) encode the modification of LPS to Lipid IVA

Figure 1:  Purity

Maintain clones at low-copy number

Non-proofreading Polymerase

forming oligoribonucleotides having 3´-terminal pyrimidine-3´-phosphates

RNase I, 1,000 U size:EACH Seven specific deletion mutations (ΔgutQRemove unwanted RNA by digesting with this heat labile, non sequence specific ribonuclease Complete: Unlike RNase A, RNase I does not have a base preference and digests between all dinucleotide pairs in single stranded RNA Heat Labile: RNase I is easily inactivated by heating 70C for 15 minutes RNase I degrades single stranded RNA to nucleoside 3 monophosphates via 2,3 cyclic monophosphate intermediates by cleaving between all dinucleotide pairs,2,3