Southern Blot Analysis size:For class demonstration. Use of EpiScript® Reverse Transcriptase

Use of EpiScript® Reverse Transcriptase for first strand cDNA results in detection of similar transcript categories independent of input amount

Specific: Processively digest ssDNA in a 3´ to 5´ direction without harming dsDNA - short 3´ overhangs in DNA are not digested

Assisted GeneFiX™ DNA Saliva Collector for 2 samples

100-bp ladder

the greater the amount of residual DNA not digested by the indicated DNase

Southern Blot Analysis size:For class demonstration. Use of EpiScript® Reverse TranscriptaseSouthern blot analysis, named after its inventor Edwin M. Southern, is a common research technique for enhancing the results of agarose electrophoresis. DNA is resolved on an agarose gel and is subsequently transferred to a nylon membrane. The resulting membrane can be probed for specific DNA sequences using a single stranded probe that anneals or hybridizes with a specific sequence. The hybridization probes are often labeled, which can be detected