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The approximately 2160-base HeLa β-glucuronidase mRNA (GUSB) was reverse transcribed into cDNA using Epicentre's MMLV High Perfomance Reverse Transcriptase and two competitive reverse transcriptase enzymes
RNase protection assays to detect single-basepair mismatches in RNA:RNA and RNA:DNA hybrids
such as polysaccharide
1-kb clone directly from a ligation reaction 2
Unit Definition: One unit of CircLigase enzyme converts 1 pmol of a linear 5´-phosphorylated CircLigase Standard 55-mer Oligo into an exonuclease I-resistant circular form in 1 hour at 60°C under standard assay conditions
SP6 RNA Polymerase (5000U) - 5000U size:5000U The approximately 2160-base HeLa β-glucuronidase
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