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possesses greater strand-displacing polymerase activity
Improved success in demanding downstream applications
we recommend the MasterAmp™ PCR Optimization Kit with ammonium sulfate for rapid PCR optimization
Preserves Yields
Specific: Digests ssDNA in the 5' to 3 direction
Exonuclease VII, 250 U @ 10 U/uL ISOBUC possesses greater strand-displacing polymerase activityDigest duplex DNA in a 3 to 5 direction from a nick, blunt end, or 3 recessed end, producing stretches of ssDNA. Produce stretches of ssDNA in dsDNA templates containing nicks, blunt ends or recessed 3' ends Protect protruding 3' ends becuase this exonuclease will not start digestion at protruding 3' overhangs Applications Production of intermediates for site directed mutagenesis. 1 3 Production of strand specific radiolabeled probes. 4 Exonuclease
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