E. cloni-EXPRESS BL21(DE3)Chemically Competent Cells BAC-Optimized Replicator Poly(A) Polymerase uses ATP as

Poly(A) Polymerase uses ATP as a substrate for template-independent addition of adenosine monophosphate to the 3´-hydroxyl termini of RNA molecules

Unit Definition: One unit of MasterAmp Tth DNA Polymerase converts 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 70°C under standard assay conditions

Simple: No centrifugation steps of spin columns used to help increase yields

500 ng of uncut plasmid DNA

Richardson

E. cloni-EXPRESS BL21(DE3)Chemically Competent Cells BAC-Optimized Replicator Poly(A) Polymerase uses ATP asE. cloni EXPRESS BL21(DE3) Competent Cells Best value for routine protein expression available as Electrocompetent and Chemically Competent Cells. Lucigens E. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25 1,000 fold relative to competitors preparations of BL21 cells (Figure 1), which is essential for construction of