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It requires: verifying the chemical compatibility between the peptides (cross-stability), optimizing the ratio to maintain the bioactivity of each component, individual quality control of each peptide before co-formulation, and validating the stability of the lyophilized mixture over time

Hypoxic mice were administered hypoxic treatment in a closed hypoxic chamber (China Innovation Instrument Co., Ltd, Ningbo, Zhejiang, China), which accurately set the desired hypoxic concentration and pattern

Transepidermal water loss (TEWL) increases

Western blot band intensities were quantified with n = 3 by densitometry using ImageJ (Fiji) and normalized to the corresponding loading control (-actin)

Finally, select an injection site, commonly the upper arm, thigh, or abdomen, and clean it with an alcohol swab

10.1007/s00109-016-1488-y [DOI] [PubMed] [Google Scholar] Huang, T