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For determination of total glutathione, the untreated, SSA-diluted liver or bile samples were first diluted by an additional 500 fold using a phosphate (100 mM)-EDTA (1 mM) buffer (pH 7.4) before analysis

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Part III of the scale is a clinical evaluation of motor symptoms with score changes from full OFF to full ON exceeding 10 or more points [21, 22]

Li J, Lama R, Galster SL, Inigo JR, Wu J, Chandra D, et al

This is what makes once-weekly dosing possible and what makes the compound so effective compared to the naturally occurring hormone