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Every 24 h a biofilm was removed, and quantification of viable bacteria within the biofilm (CFU/ml) was calculated by vortexing the biofilm with sterile 3 mm glass beads (Merck-Millipore, Watford, UK) in Dey-Engley neutralising broth (Merck-Millipore), before serial diluting (10 1 to 10 7 )

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