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Systemic depletion of L-cyst(e)ine with cyst(e)inase increases reactive oxygen species and suppresses tumor growth

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The column was extensively washed with the wash buffer (50 mM HEPES-NaOH pH 8.0, 300 mM NaCl, 10% glycerol, 1 mM TCEP), and elution was performed with wash buffer containing 10 mM desthiobiotin

Metabolic coupling and the Reverse Warburg Effect in cancer: Implications for novel biomarker and anticancer agent development