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Isopropyl -d-1-thiogalactopyranoside (IPTG) was added to a final concentration of 1 mM and the culture was further incubated at 18 C overnight

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In addition, the levels of the adipokines C1q/TNF-related protein 2 (CTRP2) and C1q/TNF-related protein 9 (CTRP9), and the growth factors growth differentiation factor 8 (GDF8) and growth differentiation factor 15 (GDF15) were significantly reduced [10]

Frequency and dosage depend on factors like medical condition, response to treatment, age, diet, and overall health