10.13193/j.issn.1673-7717.2025.07.025 Chin Arch Trad Chin Med
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Gels were subsequently stained by Instant Blue (Expedeon)
Enzymatic reactions were performed in 96-well plate format (Greiner Bio-One, 655101) with 190 l of reaction mix and 10 l of enzymes diluted in 10 mM sodium phosphate buffer pH 7.0 at the following concentrations: 1.36 g ml 1 (LacAnc100) and 0.81 g ml 1 (LaccID) for ABTS, 1.7 g ml 1 (LacAnc100) and 0.81 g ml 1 (LaccID) for DMP and guaiacol and 6.8 g ml 1 (LacAnc100) and 8.1 g ml 1 (LaccID) for sinapic acid
Database searching was performed in Proteome Discoverer (PD, Thermo Fisher Scientific) v3.0 against the UniProt human reference proteome, allowing tryptic peptides (730 aa), up to two missed cleavages, and up to three variable modifications per peptide
Symptoms include high blood pressure, increased body fat, high blood sugar, dyslipidemia, and problems with cholesterol and triglycerides
