Glutathione-sensitive linkers rely on the difference in redox environments between the inside and outside of cells to achieve drug release
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Often used in GHK-Cu peptide injection protocols that target wrinkles and skin laxity
While previous research has predominantly focused on the role of individual proteins in response to H 2 O 2 exposure [16, 24], only one proteomic study examined the global changes in chondrocytes exposed to both H 2 O 2 and hyaluronic acid in 2014, identifying a limited number of protein targets [25]
Two different reaction mixtures were prepared for each sample to assess hPrx2 and cysteinylated hPrx2 activity: 1 M Prx2 or 1 M Prx2-S-S-Cys (cysteinylated Prx2), 1 M Trx1, 2 nM TrxR1, 300 nM NADPH, and 100 mM H 2 O 2 in TE buffer (50 mM Tris, 1 mM EDTA)
Running multiple peptides simultaneously represents a significant ongoing expense