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Notably, the affinity of cysteine for cysteinyl-tRNA synthetase is orders of magnitude higher than its affinity for the rate-limiting enzyme in GSH synthesis (GCLC) 48,49
Cells generated at different stages of the differentiation process, such as DE cells and HLCs, were fixed with 4% PFA for 20 min at room temperature, rinsed with DPBS with Ca2+ and Mg2+, and permeabilized with 0.3% Triton X-100 in DPBS for 15 min
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