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Given that many phytochemicals act on overlapping or converging molecular targets (e.g., PI3K/Akt, BMP2, Wnt), potential synergistic or antagonistic crosstalk may significantly influence the osteoangiogenic coupling process

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GussJDZiemianSNLunaMSandovalTNHolyoakDTGuisadoGGet al

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Additionally, single-point alanine mutants within conserved residues of the CPD motif, specifically T18A, P19A, and S22A, were also created

While a substitution with the polar serine at position 13 retained wild-type dehydratase activity, presumably due to the alcohols ability to assume cysteines function in stabilizing deprotonated GS , the only other Pt GSTF8 variants to keep analbeit reducedflavan-3,3,4-triol ( 4 ) dehydration activity contained a glycine, alanine or asparagine mutation (Fig