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31 The lower H 2 O 2 levels in SHR in this study seems to suggest that H 2 O 2 might not have a significant direct role in the pathogenesis of hypertension in this strain of rat as despite the lower H 2 O 2 levels, systolic blood pressure still increased in the SHR

The primers used were 5-caccatcacctcctggaaga-3 and 5-gggtgcagaatctcgagttg-3 for Pck1 , 5-gtggcagtggtcggagact-3 and 5-acgggcgttgtccaaac-3 for G6pc , 5-tacagagtgctggccaagag-3 and 5-ttcaaggatgcactggagag-3 for Cyp7a1 , 5-tagccctctttcctccactcata-3 and 5-gaagcgatcgaacctaaattcct-3 for Cyp7b1 , 5-gcctcacctatgggatcttca-3v and 5v-tcaaagcctgacgcagatg-3v for Cyp27a1 , 5-ggctggcttcctgagcttatt-3 and 5-acttcctgaacagctcatcgg-3 for Cyp8b1 , 5-acctgtctaacctcttcacc-3 and 5-caatgctgaggttcatgtcc-3 for Slc10a1 , 5-ggaagattgttggcccgatt-3 and 5-agtgggagttatggtcaggt-3v for Slco1a1 , 5-gcgtcgtgattagcgatga-3 and 5-atggcctcccatctcctt-3 for Hprt

Duggan, M

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