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Never freeze reconstituted solution

Topical antibiotics like metronidazole and erythromycin are the primary prescription treatments, working through anti-inflammatory rather than antibacterial mechanisms

Eluted proteins were concentrated using an Amicon Ultra-15 Centrifugal Filter Unit (10 kDa cutoff) and further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 gel filtration column (Cytivia 28990944) in TBS buffer

This also raises an unresolved question as to whether GSH efflux serves primarily as a permissive biochemical event or represents an actively regulated signaling step in apoptotic commitment

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Mass data, including the retention time, m / z , and ion intensities, were extracted using Progenesis QI software (Waters)