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The critical transition point is the first needle puncture

The first step is to perform a glutamate dehydrogenase (GDH) assay and then an enzyme immunoassay (EIA) for toxins A and B

The dose-response curve between 8 and 12 mg shows diminishing returns, meaning 10 mg captures most of the benefit while potentially reducing some of the dose-related side effects seen at 12 mg

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Once reconstituted with bacteriostatic water or appropriate solvent, the solution should be stored at 2-8C and used within the recommended timeframe