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The AP is part of the dorsal vagal complex, closely associated with baroreceptors and chemoreceptors, as well as cranial nerves VII and IX (see Figure 3 )

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5,6,7 GLP-1 is a peptide produced by the cleavage of proglucagon, mainly synthesized in the intestinal mucosal L-cells, pancreatic islet -cells, and neurons in the nucleus of the solitary tract

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Concurrently, a reduction in the diversity of the intestinal microbiota was observed, characterized by a decrease in the relative abundance of Bacteroidetes and an increase in Proteobacteria

The abdomen also tends to have less nerve density than the thigh at the specific insertion points used, which translates to less sensation during injection