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medium in which NCs or NC-containing tissue is cultured for 4 days and comprises NC-secreted soluble factors) has the potential to increase NP GAG production and cell proliferation [6163]

This subtracted library was added to a monolayer of Ishikawa cells grown in a 3.5-cm tissue culture plate and incubated at 37 C for 30 min, allowing bound phage to be internalized by endocytosis

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