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3D culture models with CRISPR screens reveal hyperactive NRF2 as a prerequisite for spheroid formation via regulation of proliferation and ferroptosis

In C2C12 myotubes, NNMT inhibitor treatment produces concentration-dependent increases in NADH levels (50% higher at 30 M) and reduces NAD+/NADH ratios by 25-40%

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PMID 24470095