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5.4 Experimental validation of epigenetic mechanisms Recent technological advances in genome editing and single-cell analysis have provided powerful tools to validate EED-induced epigenetic modifications as causal mechanisms underlying transgenerational reproductive toxicity

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In one study, approximately one in every three people with depression had elevated homocysteine levels

However, there is still a lack of in vivo understanding of many processes at neural barriers, making robust validation of model systems to be associated with noticeable difficulties

doi: 10.1016/S0140-6736(21)00136-7 22 NiemanLKChanco TurnerML

however, individual responses vary and monitoring is important