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[DOI] [PMC free article] [PubMed] [Google Scholar] 132.Li J., Lei J., He L., Fan X., Yi F., Zhang W

The first step in methionine metabolism is the formation of S-adenosylmethionine (SAMe) in a reaction catalyzed by methionine adenosyltransferase (MAT) (Finkelstein, 1990

Cells were collected after 72 h and lysed by gentle nutation in wash buffer (50 mM HEPES, 150 mM NaCl, 2.5 mM MgCl 2 , 1 mM TCEP, pH 7.4) supplemented with 0.4% CHAPS and Protease Inhibitor (Roche) for 1 h

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