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Medium was replaced with Seahorse XF Base Medium (Agilent) supplemented with 15 mM glucose (Gibco, A2494001), 1 mM sodium pyruvate (Gibco, 11360070), and 4 mM L-glutamine (Gibco, 25030081), pH 7.4, unless indicated otherwise and incubated in a non-CO2 incubator at 37 C for less than one hour

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It increased bioavailability and protection against degradation, making it a potential substitute delivery method for oral EGCG [37]

10.1093/bioinformatics/btu301 Bioinformatics 29 ChiuehC

However, it is important to note that B complex injections are usually given as a one-time treatment or as part of a short-term treatment plan, rather than as a long-term supplementation strategy